- PII
- S3034551025060044-1
- DOI
- 10.7868/S3034551025060044
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 70 / Issue number 6
- Pages
- 917-924
- Abstract
- Using the method of small-angle X-ray scattering (SAXS), the temperature dependencies (in the range of 4 to 30°C with a 1°C step) of the structure of a lysozyme solution during the growth of tetragonal crystals were obtained for HO and DO solvents. It was found that, regardless of the solvent type, dimers and octamers form in the lysozyme crystallization solution. The volume fractions of the oligomers (dimers and octamers) are inversely proportional to the temperature change, both in the case of the HO solvent and the DO solvent. Under identical temperature conditions, the volume fraction of oligomers in the lysozyme crystallization solution in DO is approximately 8% higher than in HO. However, the same oligomer content in the lysozyme crystallization solutions in HO and DO is achieved when the temperature of the HO solution is approximately 10°C lower than that of the DO solution. This may be explained by the distinct influence of the solvent on protein hydration.
- Keywords
- Date of publication
- 15.09.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 4
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