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Temperature influence on the stability of the precursor cluster of the thermolysin crystal
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Temperature influence on the stability of the precursor cluster of the thermolysin crystal
Annotation
PII
S0023476124040165-1
Publication type
Article
Status
Published
Authors
Y. V. Kordonskaya 
Occupation: Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics
Affiliation:
National Research Centre “Kurchatov Institute”
NRC “Kurchatov Institute”
Edition
Volume 69 Issue number 4
Pages
694-699
Abstract
We used the molecular dynamics method to assess the stability of the precursor-cluster (hexamer) of thermolysin crystal over a wide range of temperatures (10–90°C). The simulation results showed that as the temperature increases, the stability of the hexamer, in general, decreases, however, the hexamer does not dissociate at any of the considered temperatures. At a temperature of 60°C, an increase in the stability of the hexamer was observed. This value is close to the temperature of maximum enzymatic activity of thermolysin (70°C). Based on the analysis of the results, it was assumed that the crystallization of thermolysin could be carried out at 60°C.
Acknowledgment
Ministry of Science and Higher Education (075-15-2021-1363). Government of the Russian Federation.
Received
22.09.2024
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16
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